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Webinar Videos

Azure Biosystems Live Webinar Series Beyond The Blot

Presented by Joy DeTorres, Application Scientist:
Total protein normalization is the new standard required by journals for western blot quantification. Housekeeping proteins (HKPs) are widely used as an internal reference for western blot normalization. They are often highly expressed, lose proportionality at high protein loads, and only represent one portion of your whole sample. For this reason, using HKPs is an unreliable method of calculating normalization ratios.

TotalStain Q accurately accounts for lane-to-lane variations inherent in western blotting. With linearity up to 50 μg of lysate, TotalStain Q is ideal for the normalization of lowly expressed proteins that require high sample loads. Kits are available for both PVDF and nitrocellulose membranes.

TotalStain Q is compatible with any instrument capable of green channel excitation (including the Azure Imager & Sapphire Q modules). Combine TotalStain Q with your downstream near-infrared or chemiluminescent imaging protocol for quantitative analysis, without any destaining step required. This two component, room temperature stable fluorescent stain makes total protein normalization both easy and efficient.

 

Azure Biosystems Live Webinar Series Beyond The Blot

Presented by: Dr. Brian Bauereis, Field Application Scientist:
At Azure Biosystems we design and manufacture novel lab instrumentation to generate and analyze images of a wide range of assays. Our innovative platforms provide superior performance to a vast array of applications in bioscience. Both platforms were designed to be the most sensitive systems currently available in the market. This talk will explain how Azure has used the latest in technology to achieve such flexibility in chemistry selection and performance from a scientific and technical perspective. This talk will also focus on exciting experimental paradigms using Azure instrumentation. The presenter, Dr. Brian Bauereis, a Yale alumnus, has worked with Azure for 4 years and will present on how these advancements can benefit the scientific and medical communities.

 

Array Imaging on the Azure Sapphire Biomolecular Imager

Presented by Dr. Andreas Schrötter, Scientific Sales Manager:
Whatever type of imaging your lab does, whether it’s the ubiquitous western blot, fluorescent gels, visualizing gross morphology of tissues or something more unique, The Sapphire™ Biomolecular Imager will deliver outstanding, quantitative detection with NIR and RGB fluorescence, chemiluminescence, and phosphor imaging. While western blotting has been a lab standard for decades, the high performance of our scanner enables also time- and labor-saving extensions of the western blot. One such extension is an cell-based assay in microplates, where plate-grown cells are fixed, permeabilized, and then probed with antibody in situ. The result is an accurate measurement of intracellular protein expression while the cells are still in the plate. In this webinar we present the next generation of laser-based imaging with various types of array based applications such as cell-based assays in microplates and protein microarrays.

 

Mouse Imaging on the Azure Sapphire Biomolecular Imager

Presented by Dr. Andreas Schrötter, Scientific Sales Manager:
Multiple imaging modalities give you the freedom to do more. If you’re one of the many scientists that combines biochemical techniques such as gels and Western blots with the visualization of mouse tissue or mouse morphology, it’s time to rethink how much you really need to use a microscope. With resolution down to 10μm, The Sapphire™ Biomolecular Imager enables imaging above the subcellular level and the large scanning bed translates into a ultra-wide field of view compared to a microscope. In this webinar we present the next generation of laser-based imaging with mouse samples like brain, lung, liver and so much more!

 

Starting with Fluorescent Western Blotting

Starting with Fluorescent Western Blotting

Presented by Jan Wolfram, Director, European Sales:
What should you consider when starting with a fluorescent Western blotting method? What are differences from the traditional HRP based essays? Is it more quantifiable? Are there any beginners’ mistakes to avoid with the first run? How to make a fluorescent blot a success? These questions will be answered by this introductory webinar from Azure Biosystems.

 

Western Blot Normalization

Western Blot Normalization

Presented by Justin Menko, Technical Product Specialist:
The use of housekeeping proteins (HKPs) for the normalization of western blot data is the historical norm. With that said, numerous peer reviewed journals and the NIH have started suggesting that researchers transition to using a total protein normalization methodology. This suggestion is based on a few key variables which this webinar addresses. For those not familiar with total protein normalization or wondering if it’s time to transition away from HKPs, this webinar is for you.

 

Western Blot Troubleshooting

Western Blot Troubleshooting

Presented by Joy DeTorres, Application Scientist:
Western Blotting is a science, but creating beautiful Western blots is an art. Learn tips to improve your Western blotting technique, and troubleshoot common issues with Western blotting and blot imaging to produce clean blots and more quantitative images.

 

Plant Imaging on the Azure Sapphire Biomolecular Imager

Plant Imaging on the Azure Sapphire Biomolecular Imager

Presented by Dr. Andreas Schrötter, Scientific Sales Manager:
Think about it—one instrument, one easy and flexible data acquisition for plant samples. If you’re one of the many scientists that combines biochemical techniques such as gels and Western blots with the visualization of plant samples, it’s time to think about a large field of view scanner. With resolution down to 10μm, The Sapphire™ Biomolecular Imager enables imaging above the subcellular level and the large scanning bed translates into a ultra-wide field of view to allow you to see your plant specimen in full size. In this webinar we present the next generation of laser-based imaging with plant samples like Arabidopsis thaliana roots and seedlings, plant stem cuts, various full size pant leafs and so much more!

 

Plant Imaging on the Azure Sapphire Biomolecular Imager

Biomarker Research with the Azure Imaging System

Presented by Dr. Stephanie M. Cologna, Assistant Professor, University of Illinois at Chicago:
Biomarker research is critical to medical research and the advancement of treatment and drug development. In this webinar, Dr. Stephanie M. Cologna of the University of Illinois at Chicago discusses the differential proteomic and lipidomic signatures of Niemann-Pick Disease, Type C, and how she is combining Western blotting and mass spectrometry in her research.