Azure Flash Western Kit
Run an entire Western Blot from gel through imaging in just 2 hours
The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemiluminescent signal in less than an hour of blotting time. The included transfer buffer cuts wet transfer time down to 15 to 30 minutes for a total assay time of two hours from gel to imaging.
- Fast protocol with few hands-on steps
- Antibody stabilization for enhanced chemiluminescent signal
- Low background and increased signal-to-noise ratios
- Increased detection sensitivity and linear dynamic range
Replicate Western blots were performed using the Azure Flash Western Demo Kit and a traditional Western blot protocol. Each blot shows a dilution series of the same sample. The Azure Flash Western Demo Kit produced more sensitive detection than the traditional protocol with over 90% of antibodies (while STAT-3 and pSTAT-3 demonstrate the improved data quality possible with the Azure Flash Western Demo Kit, the HSP60 antibody is an example of an antibody with similar performance).
The Flash Western Demo Kit
Compared to a traditional Western blot protocol, the Azure Flash Western can take three days of work
down to less than 2 hours.