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cSeries™ Imagers vs. iBright® systems:
What you should know

If you’re on the hunt for a new Western blot imager and are thinking about an Invitrogen® iBright® system from Thermo Fisher Scientific, think again. At a similar price point, our cSeries™ Imagers are just as easy to use but you’ll get more sensitive detection, a wider dynamic range, and a more uniform field-of-view. And with fewer moving parts than iBright® systems, cSeries Imagers are easier to maintain.

In other words, if you care about generating reliable and reproducible (not to mention quantitative) gel and blot images, a cSeries™ Imager is the obvious choice. Even Thermo Fisher thinks so:

“With a multi-wavelength imaging system you can take advantage of the full power of WesternDot® conjugate detection…Best results for multiplex imaging are achieved using probes with broad spectral separation and an imaging platform such as Azure Biosystems® c500® or Fuji.”1

1. WesternDot® Fluorescent Immunodetection Kits: Multiplex Gel Imager Tab. ThermoFisher Scientific Website. https://bit.ly/2BAdBaM. Accessed February 14, 2019.

cSeries™ Imagers combine ease-of-use with performance

c280™ iBright® CL-1000 c600® iBright® FL-1000
Number of standard light sources 4 2 9 3
Lasers No No Yes No

Notice the bench-friendly, space-saving footprint of our cSeries™ Imagers

 

Footprint of the c600® Imager (38 cm x 55 cm x 36 cm; W x H x D) vs. the iBright® FL-1000 (dotted line; 38 cm x 60 cm x 68 cm; W x H x D)

Innovative design translates into better performance

It should be no surprise that instrument design can greatly impact instrument performance. At Azure Biosystems®, we focus on using technological innovation to deliver instruments that really impact a scientist’s work, from design choices that simplify workflows and improve performance without significantly impacting price.

Direct, mirror-free detection delivers better sensitivity

One way the design of cSeries™ Imagers delivers better performance than iBright® imagers is through our direct-mirror-free detection. We place our CCD sensor close to the sample to maximize light collection efficiency and, thus, sensitivity. In contrast, the iBright® sensor is placed much farther away from the sample, reducing sensitivity. In addition, iBright® does not directly detect light from the sample, but rather the light is reflected onto the sensor using a mirror, which further reduces sensitivity.

Light Source
Light source           Decrease in light intensity (inverse-square law)
 
 

NIR lasers increase sensitivity and reduce background

The use of laser light sources for NIR illumination in cSeries™ Systems further increases sensitivity. because lasers deliver an inherently narrower excitation band than LEDs—lasers emit a coherent, collimated beam of light—they avoid the overlap in excitation and emission signals that can occur with LED light sources. This results in ultra-low background signal, enabling faster, more sensitive NIR fluorescence detection.

led-vs-laser

NIR lasers used in cSeries™ Imagers keep signal high and background low. The robust excitation energy delivered by lasers maximizes emission strength for optimal detection sensitivity.

More about our cSeries™ Imagers

All cSeries™ instruments offer:

  • Focus-free imaging, no-positioning necessary
  • One-touch workflows—select your application and the instrument chooses the appropriate light source and filters
  • AzureSpot Analysis Software for powerful and streamlined quantitative analysis

Select instruments offer:

  • Chemiluminescent detection that’s just as sensitive as film, but easier and more quantitative
  • Two NIR lasers (660 nm and 785 nm) that keep signal high and background low
  • Efficient multiplex detection with three visible fluorescence channels and/or two NIR fluorescence channels

Ready to learn more about cSeries™ Imagers?
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Invitrogen®, WesternDot® and iBright® are registered trademarks of Life Technologies Corporation, now a part of Thermo Fisher Scientific. No endorsement is implied by the use of these marks. cSeries™ and c280™ are trademarks of and c600® and Azure Biosystems® are registered trademarks of Azure Biosystems, Inc. All rights reserved.
 
 
 
 


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Reviews of the c600® Imager from SelectScience

“This is an exceptional multi-modal imaging system. It is very sensitive, provides highly uniform images, and, importantly for a general use facility, it is VERY easy to use. I would recommend this system to a friend or colleague.”

—Seth Gammon, MD Anderson Cancer Center

 
 
“Recently bought this imager after having tested Biorad, ProteinSimple, Multiple GE machines and UVP. I was looking for the imager that gave us the best image quality for our western blots, the price was not a question. I can confidently say that this imager gave us the highest image quality and had the most functionality, on top of being one of the more affordable imagers. I am very happy with this machine and would definitely recommend it.”

—Alex Chan, USC Keck