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Tag Archives | western blotting


Studying the SARS-CoV-2 proteases with the Sapphire Biomolecular Imager

Researchers at the Wroclaw University of Science and Technology and their colleagues have recently published exciting work improving our understanding of the two proteases encoded by the SARS-CoV-2 genome, the main protease (Mpro) and the papain-like protease (PLpro). These proteases are required for viral gene expression and for viral replication, so they are attractive targets […]

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Why is Total Protein Normalization Important?

If you love Western blotting as much as we do, you’ll have noticed a growing trend toward using total protein staining rather than housekeeping proteins to normalize for load. But what does total protein normalization (TPN) involve and what advantages does it offer? Read on to find out and get your hands on a free […]

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Help! Why do my Western blots look terrible?

One of the most common questions when troubleshooting problematic Western blots is “why is the background so high?” High or uneven background on western blots doesn’t just look bad – it interferes with data analysis, making it difficult to quantify bands or compare bands between samples. There are several things you can do to reduce […]

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