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Chemiluminescent Western Blot

Secrets to Chemiluminescent Western Blotting

What you need to know to optimize your chemiluminescent Western blots Chemiluminescence remains the most frequently used method to detect target proteins on Western blots. Many reagents are commercially available for chemiluminescent detection but all share basic characteristics. The secondary antibody is labeled with an enzyme, usually horseradish peroxidase (HRP). After incubation with the secondary […]

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