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Archive | Western Blotting

Scientifically accurate atomic model of coronavirus

Intranasal administration of neutralizing IgA increases SARS-CoV-2 infection in a hamster model

Infectivity of SARS-CoV-2 is associated with high viral loads in the upper respiratory tract. Alpha and gamma immunoglobulins (IgA and IgG) both neutralize viral infections, with IgA significant in mucosal immunity and IgG predominantly affecting blood. Most studies focus on IgG in SARS-CoV-2 infection, but the role of IgA is less understood. To enter host […]

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Nice Western Blot

What is Western Blotting?

Your common Western blotting questions, answered Western blotting is a widely used analytical technique that can identify one or more specific proteins in a complex mixture of proteins. It is a powerful tool that provides information about the presence, size, and under the right conditions, even the amount of a protein. Though commonly used and […]

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Visualization of the Covid-19 virus

Investigating S-acylation of the SARS-Cov-2 Spike Protein Leads to New Insights into Viral Infectivity

A better understanding of coronavirus biology can enable development of new antivirals to help stop COVID-19 and prevent future pandemics. In recent work, Puthenveetil et al. characterized S-acylation of the Spike (S) protein of SARS-CoV-2. This post-translational modification is known to be important to the viral replication cycle of other viruses across multiple virus families […]

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Chemiluminescent Western Blot

Beginning Chemiluminescent Western Blotting

What you need to know to optimize your chemiluminescent Western blots Chemiluminescence remains the most frequently used method to detect target proteins on Western blots. Many reagents are commercially available for chemiluminescent detection but all share basic characteristics. The secondary antibody is labeled with an enzyme, usually horseradish peroxidase (HRP). After incubation with the secondary […]

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