Top Navigation

 

Archive | Western Blotting

validateAntibodies_2

Quantitative Western Blotting: How and why you should validate your antibodies

Validating your antibodies, i.e.  confirming that an antibody recognizes your protein of interest with low cross-reactivity to other targets, is critical for ensuring consistent, reproducible results. Validation is frequently required before submission to a journal, but not everyone takes the time to validate. Or many of us assume that because we validated the antibody for […]

Continue Reading
non-specific_bands_1

Western Blot Troubleshooting: What to do about non-specific bands

Non-specific bands are a perennially frustrating problem in Western Blotting. Here are some of the different reasons you might be getting non-specific bands and tips on how to make these unwanted additions to your western blot disappear. . Incomplete Blocking One of the most common causes of non-specific bands is incomplete blocking. Blocking buffers are […]

Continue Reading
phosphoImg7

Visualizing and Quantifying phosphoproteins via Western Blotting Part 1 of 2

Western blotting is an important tool for researchers studying signal transduction and other processes where understanding the phosphorylation state of a protein is important for elucidating protein function. But some researchers are hesitant to use Western blotting to calculate the relative amount of phosphorylated to unphosphorylated protein. Here we discuss steps you can take to […]

Continue Reading
Guinea pig bone marrow sample with two megakaryocytes (arrows). Image courtesy of CDC PHIL ID#14465

New production method for lentivirus overcomes the barrier to using a promising pseudotype for transducing stem cells more effectively

What’s the problem, scientifically speaking? While hematopoietic stem and progenitor cells (HSPCs) have great potential for development into cell-based therapeutics, they are very difficult to transduce. This technical problem has slowed researchers’ ability to turn HSPCs into the next generation of medicines. What have been the technical barriers? The problem with transducing HSPCs has been […]

Continue Reading