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Pathological Signature of T Helper 17 Cells Associated with Endometriosis Identified

Nearly 10% of reproductive age women are affected by endometriosis globally, a condition in which the uterine tissue grows ectopically, resulting in pelvic pain and infertility. The peritoneal fluid (PF) surrounding ectopic endometrial tissue contains T helper 17 (Th17) cells. The number of Th17 cells in PF increases with disease severity; however, their function in […]

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Sapphire Biomolecular Imager Used in Landmark Study of DNA Repair Pathway

The non-homologous end-joining (NHEJ) pathway is one of two pathways responsible for repairing double-stranded breaks in cellular DNA. Double-stranded breaks can be formed by ionizing radiation or mutagenic chemicals, or as intermediates during normal processes such as the recombination of DNA segments required to create antibody and T cell receptor diversity. In contrast to homologous […]

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New method effectively stains apoptotic retinal cells without requiring intraocular injections

Azure Biosystems is excited to announce the first published study that uses the Sapphire Biomolecular Imager, and we look forward to the role that Sapphire will play in future studies. What’s the problem, scientifically speaking? Labeling neurons is critical for the diagnosis, prognosis, and treatment of retinal degenerative diseases. A common approach is to use […]

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Figure 1. AzureSpot Multiplex Image

Visualizing and Quantifying phosphoproteins via Western Blotting Part 2 of 2

Best practices for quantitation of unphosphorylated vs phosphorylated forms In part 1 we reviewed tips for using multiplexing to visualize phosphoproteins, and in part 2 we’re going to cover quantitation. Once you have reliable, repeated samples that fluoresce in non-overlapping bands and are linearly proportional to the signal, you can begin analysis. Here we will […]

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