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Total Protein Normalization: Which Imager Should You Choose?

Total protein normalization (TPN) has become the preferred method for normalizing Western blot data. But to fully leverage its benefits, you need an imaging system that allows you to multiplex TPN with detection of your target proteins. Fortunately, we have just what you need! Whether you want to combine TPN with near infrared (NIR) fluorescent […]

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Handy Resources to Improve Your Western Blots

At Azure Biosystems we live and breathe all things Western blotting. From innovative imagers, to top notch reagents and accessories, to customer education, we are here to support you in your quest for the most robust Western blotting protocols, techniques and data no matter where you are in your Western blotting career. Are you a […]

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Western Blot Troubleshooting: What to do about non-specific bands

Non-specific bands are a perennially frustrating problem in Western Blotting. Here are some of the different reasons you might be getting non-specific bands and tips on how to make these unwanted additions to your western blot disappear. . Incomplete Blocking One of the most common causes of non-specific bands is incomplete blocking. Blocking buffers are […]

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Visualizing and Quantifying phosphoproteins via Western Blotting Part 1 of 2

Western blotting is an important tool for researchers studying signal transduction and other processes where understanding the phosphorylation state of a protein is important for elucidating protein function. But some researchers are hesitant to use Western blotting to calculate the relative amount of phosphorylated to unphosphorylated protein. Here we discuss steps you can take to […]

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