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Archive | Quantification

GETTING E. COLI TO MAKE PROTEIN

A new mechanism by which bacteriophage T5 inhibits growth of E. coli

How can we better control pathogenic bacteria? Insights may come from studying bacteriophages, viruses that infect bacteria. There are a wide variety of bacteriophages, each of which is specialized to infect and replicate within a specific target bacteria. Learning how a bacteriophage takes over bacterial metabolism to direct resources towards generating more bacteriophage can both […]

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Insight into Hepatitis B virus replication gained from phosphor imaging of Southern blots with the Sapphire Imager

In a recent publication, Ji Xi et al examined the role of the linker region of the Hepatitis B virus (HBV) core protein, a protein essential for viral replication. The C-terminal domain of the core protein contains multiple phosphorylation sites that are dynamically phosphorylated and dephosphorylated by cellular kinases and phosphatases throughout the viral life […]

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Quantitative Western Blotting: How and why you should validate your antibodies

Validating your antibodies, i.e.  confirming that an antibody recognizes your protein of interest with low cross-reactivity to other targets, is critical for ensuring consistent, reproducible results. Validation is frequently required before submission to a journal, but not everyone takes the time to validate. Or many of us assume that because we validated the antibody for […]

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