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Loading control example

Loading controls and antibody validation for Western blot quantification

Today’s post is inspired by a conversation I had with a colleague when teaching them SDS-PAGE and Western blotting. Being well versed in qRT-PCR (R) and flow cytometry (R) they were particularly interested in how and why certain loading controls were used, and what other techniques were used to validate loading, changes in protein expression […]

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Bendy bands and what to do with them

If you’ve worked with SDS-PAGE and Western blotting for a while you’ll undoubtedly have seen bendy or fuzzy bands, so unlike those perfect razor-sharp rectangles shown on your antibody datasheet. While unattractive in their own right these bendy bands also make determining the molecular weight difficult, can interfere with band densitometry analysis, especially when using […]

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