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Black tube sponges

The mechanism of action of a potential anti-cancer therapeutic: new technique to monitor subcellular environments

Callyspongiolide (CSG), derived from marine sponges, is highly toxic to human cancer cell lines from a variety of origin tissues and presents a potential cell-killing agent to be included in antibody-drug conjugates for anti-cancer therapeutics. CSG-mediated cell death doesn’t appear to occur via the canonical apoptosis pathway because cell death is not blocked by caspase […]

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ICW

Western Blots Step Aside – Use Cell-Based Assays to Detect Immunofluorescence in 384-Well Plates

Although Western blotting is an established technique for detecting a specific protein within a complex sample, it has several inherent shortcomings. These include a lengthy workflow, low throughput, and limitations in the number of replicates, leading many researchers to seek an alternative method. This is where quantitative immunofluorescence in tissue culture comes in. Despite not […]

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Western Blotting Reagents Roundup – Recent Publications

Azure offers products to support every step of your Western blotting workflow, from blocking to detection. Several recent publications highlight the use of Azure reagents in chemiluminescent and fluorescent Western blotting, in a variety of experimental systems and approaches. Below is a brief summary of four articles in which researchers incorporated Azure’s protein-free blocking buffer, […]

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Figure 1. AzureSpot Multiplex Image

Visualizing and Quantifying phosphoproteins via Western Blotting Part 2 of 2

Best practices for quantitation of unphosphorylated vs phosphorylated forms In part 1 we reviewed tips for using multiplexing to visualize phosphoproteins, and in part 2 we’re going to cover quantitation. Once you have reliable, repeated samples that fluoresce in non-overlapping bands and are linearly proportional to the signal, you can begin analysis. Here we will […]

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