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Archive | In Cell Western

IMG-1634

Customer Interview – Using the Azure c600 to Study Innate Immunity – Thiago Soares, PhD (National Institutes of Health, Bethesda, MD)

“I instantaneously got excited about the [Azure c600]: the layout was very simple, it was compact, and had a user-friendly software– so for me, it was very convenient.”   What is your current position? I have been a Postdoctoral Fellow at the NIH/NIAD, in Bethesda, MD for the past 3.5 years. Before, I worked as […]

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ICW

Western Blots Step Aside – Use Cell-Based Assays to Detect Immunofluorescence in 384-Well Plates

Although Western blotting is an established technique for detecting a specific protein within a complex sample, it has several inherent shortcomings. These include a lengthy workflow, low throughput, and limitations in the number of replicates, leading many researchers to seek an alternative method. This is where quantitative immunofluorescence in tissue culture comes in. Despite not […]

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Direct Indirect

The evolution of the ELISA and other immunoassays

If you’re reading this blog there’s a high chance that you use antibodies in your work, in some form of immunoassay. However, many of us rarely stop and think about where these antibodies come from and how their development has been fundamental for the evolution of life sciences. A brief history of the antibody Antibodies […]

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