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Archive | Immunoassay

IMG-1634

Customer Interview – Using the Azure c600 to Study Innate Immunity – Thiago Soares, PhD (National Institutes of Health, Bethesda, MD)

“I instantaneously got excited about the [Azure c600]: the layout was very simple, it was compact, and had a user-friendly software– so for me, it was very convenient.”   What is your current position? I have been a Postdoctoral Fellow at the NIH/NIAD, in Bethesda, MD for the past 3.5 years. Before, I worked as […]

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validateAntibodies_2

Quantitative Western Blotting: How and why you should validate your antibodies

Validating your antibodies, i.e.  confirming that an antibody recognizes your protein of interest with low cross-reactivity to other targets, is critical for ensuring consistent, reproducible results. Validation is frequently required before submission to a journal, but not everyone takes the time to validate. Or many of us assume that because we validated the antibody for […]

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effect of blocking agent

Getting Rid of the Noise: Western Blot Blocking

Why is the Western blot blocking step important? After a sample has been transferred from the gel to a blotting membrane, most Western blotting protocols include a blocking step before incubating the blot with the primary antibody. Understanding how blocking works is key to maximizing the quality of your Western blot, improving specificity, sensitivity, and […]

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Direct Indirect

The evolution of the ELISA and other immunoassays

If you’re reading this blog there’s a high chance that you use antibodies in your work, in some form of immunoassay. However, many of us rarely stop and think about where these antibodies come from and how their development has been fundamental for the evolution of life sciences. A brief history of the antibody Antibodies […]

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