Top Navigation

 

Archive | Fluorescence imaging

retina

New method effectively stains apoptotic retinal cells without requiring intraocular injections

Azure Biosystems is excited to announce the first published study that uses the Sapphire Biomolecular Imager, and we look forward to the role that Sapphire will play in future studies. What’s the problem, scientifically speaking? Labeling neurons is critical for the diagnosis, prognosis, and treatment of retinal degenerative diseases. A common approach is to use […]

Continue Reading
Figure 1. AzureSpot Multiplex Image

Visualizing and Quantifying phosphoproteins via Western Blotting Part 2 of 2

Best practices for quantitation of unphosphorylated vs phosphorylated forms In part 1 we reviewed tips for using multiplexing to visualize phosphoproteins, and in part 2 we’re going to cover quantitation. Once you have reliable, repeated samples that fluoresce in non-overlapping bands and are linearly proportional to the signal, you can begin analysis. Here we will […]

Continue Reading
phosphoImg7

Visualizing and Quantifying phosphoproteins via Western Blotting Part 1 of 2

Western blotting is an important tool for researchers studying signal transduction and other processes where understanding the phosphorylation state of a protein is important for elucidating protein function. But some researchers are hesitant to use Western blotting to calculate the relative amount of phosphorylated to unphosphorylated protein. Here we discuss steps you can take to […]

Continue Reading
Guinea pig bone marrow sample with two megakaryocytes (arrows). Image courtesy of CDC PHIL ID#14465

New production method for lentivirus overcomes the barrier to using a promising pseudotype for transducing stem cells more effectively

What’s the problem, scientifically speaking? While hematopoietic stem and progenitor cells (HSPCs) have great potential for development into cell-based therapeutics, they are very difficult to transduce. This technical problem has slowed researchers’ ability to turn HSPCs into the next generation of medicines. What have been the technical barriers? The problem with transducing HSPCs has been […]

Continue Reading