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Deciphering Lipid Metabolism in Borrelia burgdorferi (Bb) to Better Understand Lyme Disease

Lyme disease is the most common tick-born disease in the United States, with almost half a million cases diagnosed every year. Lyme disease is caused by the bacterium Borrelia burgdorferi (Bb), which is transmitted to humans when they are bitten by an infected tick. Bb does not produce any toxins, and the pathogenesis of Lyme […]

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Western Blots Step Aside – Use Cell-Based Assays to Detect Immunofluorescence in 384-Well Plates

Although Western blotting is an established technique for detecting a specific protein within a complex sample, it has several inherent shortcomings. These include a lengthy workflow, low throughput, and limitations in the number of replicates, leading many researchers to seek an alternative method. This is where quantitative immunofluorescence in tissue culture comes in. Despite not […]

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Western Blotting Reagents Roundup – Recent Publications

Azure offers products to support every step of your Western blotting workflow, from blocking to detection. Several recent publications highlight the use of Azure reagents in chemiluminescent and fluorescent Western blotting, in a variety of experimental systems and approaches. Below is a brief summary of four articles in which researchers incorporated Azure’s protein-free blocking buffer, […]

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Quantitative Western Blotting: How and why you should validate your antibodies

Validating your antibodies, i.e.  confirming that an antibody recognizes your protein of interest with low cross-reactivity to other targets, is critical for ensuring consistent, reproducible results. Validation is frequently required before submission to a journal, but not everyone takes the time to validate. Or many of us assume that because we validated the antibody for […]

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