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Fluorescence Western Blotting

Multiplex Westerns – Insider tips

Here at Azure Biosystems we’re great believers in the power of fluorescent multiplexing in Western blots, which has driven a lot of the development of our cSeries imagers. But we recognize that taking the step from a standard HRP/chemiluminescent based approach can be daunting, and so we’ve come up with 6 tips to make the […]

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One membrane to rule them all

While we might not like to admit it us scientists are creatures of habit, reducing variables to ensure our hypothesis testing is as accurate as possible rapidly creeps into our day to day lab tasks. In some ways this can be good, with standard operating procedures and recipes being handed down through the lab to […]

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Bendy bands and what to do with them

If you’ve worked with SDS-PAGE and Western blotting for a while you’ll undoubtedly have seen bendy or fuzzy bands, so unlike those perfect razor-sharp rectangles shown on your antibody datasheet. While unattractive in their own right these bendy bands also make determining the molecular weight difficult, can interfere with band densitometry analysis, especially when using […]

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Western Blotting