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Figure 1. AzureSpot Multiplex Image

Visualizing phosphoproteins via Western Blotting Part 2 or 2

Best practices for quantitation of unphosphorylated vs phosphorylated forms In part 1 we reviewed tips for using multiplexing to visualize phosphoproteins, and in part 2 we’re going to cover quantitation. Once you have reliable, repeated samples that fluoresce in non-overlapping bands and are linearly proportional to the signal, you can begin analysis. Here we will […]

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phosphoImg7

Visualizing and Quantifying phosphoproteins via Western Blotting Part 1 of 2

Western blotting is an important tool for researchers studying signal transduction and other processes where understanding the phosphorylation state of a protein is important for elucidating protein function. But some researchers are hesitant to use Western blotting to calculate the relative amount of phosphorylated to unphosphorylated protein. Here we discuss steps you can take to […]

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Guinea pig bone marrow sample with two megakaryocytes (arrows). Image courtesy of CDC PHIL ID#14465

New production method for lentivirus overcomes the barrier to using a promising pseudotype for transducing stem cells more effectively

What’s the problem, scientifically speaking? While hematopoietic stem and progenitor cells (HSPCs) have great potential for development into cell-based therapeutics, they are very difficult to transduce. This technical problem has slowed researchers’ ability to turn HSPCs into the next generation of medicines. What have been the technical barriers? The problem with transducing HSPCs has been […]

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Chemiluminescent Western Blot

Secrets to Chemiluminescent Western Blotting

What you need to know to optimize your chemiluminescent Western blots Chemiluminescence remains the most frequently used method to detect target proteins on Western blots. Many reagents are commercially available for chemiluminescent detection but all share basic characteristics. The secondary antibody is labeled with an enzyme, usually horseradish peroxidase (HRP). After incubation with the secondary […]

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