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Doxorubicin-DNA complex

Detecting a fluorescent anti-cancer drug using the Azure c600 imager

In a recent publication researchers used the fluorescence imaging capacity of the Azure c600 imager in a creative way, detecting the inherent fluorescence of an anti-cancer drug, as part of their investigation into a novel anti-cancer treatment. Prostate-Specific Membrane Antigen-Targeted Site-Directed Antibody-Conjugated Apoferritin Nanovehicle Favorably Influences In Vivo Side Effects of Doxorubicin. Dostalova S, et […]

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Direct Indirect

The evolution of the ELISA and other immunoassays

If you’re reading this blog there’s a high chance that you use antibodies in your work, in some form of immunoassay. However, many of us rarely stop and think about where these antibodies come from and how their development has been fundamental for the evolution of life sciences. A brief history of the antibody Antibodies […]

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Linear range of detection and what it means for your quantifications

When quantifying Western blots many people assume it’s ok to simply image your protein of interest, strip, re-probe for their loading control of choice and then use this to normalise, without considering the linear range of detection. However, there are several pitfalls associated with this, and more and more journals are now asking for a […]

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Fluorescence imaging
In Cell Western
Protein Assays
Western Blotting