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Western Blotting

Azure Imager c300

Azure Imager c300

Chemiluminescent Western Blotting

Using a digital imager like the c300 instead of film for your chemiluminescent blots gives you wider dynamic range, more accurate quantitation, reduces use of harmful chemicals, and streamlines your protocol from detection to analysis. The c400, c500, and c600 can also detect chemiluminescence. Chemiluminescent western blotting is the preferred Western blotting technique for high sensitivity and single protein detection.

Related literature:
Why You Should Leave the Darkroom (PDF)
How to Improve Your Chemiluminescent Western Blots (PDF)



azure-c400-c600

Azure c400 – c600

Fluorescence Western Blotting

Using visible fluorescent dyes, detect up to three proteins at once on the c400 imager. The c500 has IR imaging capabilities, and the c600 has the most flexibility with both IR and RGB detection. Fluorescent western blotting is the best option for quantitative comparisons between protein samples and for multiple protein detection.

Related literature:
Azure Biosystems Presents the Only Imaging System for all Western Blot Applications (PDF)
Tips for Transitioning from Chemiluminescent to Fluorescent Western Blotting (PDf)



Reagents and Accessories

Reagents and Accessories

Azure supports your Western blotting workflow with both chemiluminescent and fluorescent Western blotting reagents and accessories.

Related literature:
Western blotting workflow brochure (PDF)