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Total Protein

AO Microplate Reader

Ao Absorbance Microplate Reader

Bradford or Lowry Assays can give you total protein concentration

A convenient and fast method for measuring the protein concentration of many samples is to use a 96-well plate and the Ao Absorbance Microplate Reader and Bradford or Lowry assays. In these assays, the relative concentration of protein in a solution is proportional to a change in absorbance.

Related literature:
Total Protein by Quantification by Bradford using the Azure Ao Absorbance Microplate Reader (PDF)
Three-Color Western Blots with AzureSpectra Antibodies (PDF)
Imaging Three-color Western Blots with the Azure c600 (PDF)

Fluorescent Protein Stains

Azure 400 – 600 Imagers

Sapphire Biomolecular Imager

Fluorescent protein stains allows visualization of total protein on a gel or membrane

Try the AzureRed protein stain if you want to verify the purity of a protein after a purification procedure, to look at protein cleavage or verify proteolysis, and other applications where the blotting and antibody hybridization steps may be unnecessary. Then, image and analyze easily with the 400 or 600 digital imager.

AzureRed can also be used as a membrane stain for total protein normalization. Membranes can be stained prior to performing a Western blot, and total protein signal can be detected simultaneously with proteins of interest using the Sapphire Biomolecular Imager – NIR-Q, RGB or RGBNIR.

Related literature:
Accurate Western Blot Normalization with AzureRed Fluorescent Protein Stain (PDF)