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Total Protein

AO Microplate Reader

Ao Absorbance Microplate Reader

Bradford or Lowry Assays can give you total protein concentration

A convenient and fast method for measuring the protein concentration of many samples is to use a 96-well plate and the Ao Absorbance Microplate Reader and Bradford or Lowry assays. In these assays, the relative concentration of protein in a solution is proportional to a change in absorbance.

Related literature:
Total Protein by Quantification by Bradford using the Azure Ao Absorbance Microplate Reader (PDF)
Three-Color Western Blots with AzureSpectra Antibodies (PDF)
Imaging Three-color Western Blots with the Azure c600 (PDF)




Fluorescent Protein Stains

Azure c400 – c600

Fluorescent protein stains allows visualization of total protein on a gel or membrane

Try the AzureRed protein stain if you want to verify the purity of a protein after a purification procedure, to look at protein cleavage or verify proteolysis, and other applications where the blotting and antibody hybridization steps may be unnecessary. Then, image and analyze easily with the c400 or c600 digital imager.

Related literature:
Imaging In-Gel Fluorescence and Stain-Free™ Gels with the Azure c600 (PDF)
Stain-Free™ Gels as Part of the Western Blotting Workflow: Imaging and Total Protein Normalization (PDF)