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Multiplex Protein Detection


Azure 400 – 600 Imagers

Fluorescence Western Blotting allows detection of up to three proteins at once

Fluorescent western blotting is the best option for multiple protein detection. Antibodies conjugated with different fluorescent dyes allow for simultaneous detection of different target proteins on a blot. As long as the fluorophores have different excitation and emission spectral characteristics, up to three colors can be detected at the same time.

Related literature:
Three-Color Western Blots with AzureSpectra Antibodies (PDF)
Imaging Three-color Western Blots with the Azure c600 (PDF)

Reagents and Accessories

Reagents and Accessories

For multiplex detection experiments, it can be convenient to use antibodies already validated by your lab; use AzureSpectra fluorescent antibody labeling kit to prepare primary or secondary antibodies conjugated with the fluorophore of your choice.

Azure also provides fluorescent secondary antibodies, fluorescent Western blot buffers, opaque incubation trays, and other products that can help you on your way to getting the best multiplexing data.

Related literature:
Western blotting workflow brochure (PDF)