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Gel Documentation

Gel Documentation

Azure Imager c150-200

Imaging DNA and Protein Gels

Nucleic acid and protein gels are a fundamental part of lab life. DNA or RNA is usually stained by ethidium bromide, which intercalates into the nucleic acids and can be visualized with UV light. Safer dyes for staining DNA (SYBR® Safe, SYBR® Gold, SYBR® Green) are also growing in popularity, and can imaged with blue light.

Protein purification procedures often rely on verifying purity by SDS-PAGE and subsequent gel staining (Coomassie blue or silver stain) to check for contaminating protein bands.

The c150 and c200 imagers have the imaging capabilities for all of these applications. With a dual-wavelength 302 nm and 365 nm UV transilluminator, ethidium bromide-stained DNA gels can be imaged in a fraction of a second. For excising bands from a gel, the UV transilluminator platform can be pulled out.