Ao Absorbance Microplate Reader
ELISAs detect native antigen – antibody interactions
Enzyme-linked immunosorbent assays (ELISAs) are immunoassays where a purified sample or heterogeneous mixture of proteins or other antigen is immobilized on the surface of a plate followed by hybridization with antibodies for detection. The antibody is linked to an enzyme that can cause a color change when a substrate is added, allowing detection with a plate reader like the Ao Absorbance Microplate Reader. For detection, HRP-secondary antibodies like those for chemiluminescent Western blots can even be used.
ELISAs can be used to look for the presence or absence of a protein within a mixture, to compare relative amounts of a protein, or for other experiments that rely on native protein-antibody interactions.