Bacterial Plate Imaging

Azure Imager c400, c600

Image fluorescent bacterial colonies

Digital imagers like the c400 can be used for more than just gels and membranes. Other sources of fluorescence on a flat surface can be captured by the imager, including bacterial colonies on a plate. Fluorescent proteins like GFP variants fall in the visible range, meaning the c600 is another option as a digital imager.

Examples of when this might be useful include: on-plate screening for gene insertions or mutations, protein-protein interaction studies, gene transfer studies, characterization of colony morphology, or verification of fluorescent protein expression.

Bacterial plate imaging with the c600

The image to the left shows an agar plate with E. coli expressing GFPmut3 (green) and mCherry (red). The plate was imaged using red and green LEDs in the c600.